A New Annulenol from Aconitum heterophyllum with Bioactivity and Cytotoxicity against Vero and Lung Cell Line

In this research work, a detailed phytochemical and biological investigation of Aconitum heterophyllum was accomplished. The cold methanolic root extract A. subjected to VLC for fractionation over silica gel, which led the isolation two compounds 1,1,8,8-trimethyl-4-methylene-decahydro- 1H-cyclopenta-[8]-annulen-1-ol (1) β-stigmasterol (2). were characterized by analysis their 1D 2D NMR spectral data. Compound 1 is first reported from plant. crude screened antimicrobial activity against several gram-positive gram-negative bacteria, fungi using disk diffusion method where significant inhibitory observed. also tested cytotoxic property brine shrimp lethality bioassay, revealed moderate in comparison with standard anticancer drug, vincristine sulphate. Antioxidant methanol determined scavenging DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical found antioxidant (IC50 = 53.25 μg/mL) when compared antioxidant. Total phenolic flavonoid content polyphenolic compound HPLC. isolated 2 showed dosedependent antiproliferation vero lung A549 cells. Dhaka Univ. J. Pharm. Sci. 22(1): 1-10, 2023 (June)